![]() PCR products were subjected to gel electrophoresis at 80 V in a 1.5% agarose gel, stained with gel red, and observed using an ultraviolet transilluminator. PCR amplification was performed on a Peltier 200 Thermocycler (MJ Research, Watertown, MA) following the conditions of O’Dwyer et al. PCR amplification reactions were carried out in a final volume of 25 μL, containing 1 μL each of 10 pmol primers, 12.5 μL of Master Mix MyFi™ Mix Bioline ®, and 5 μL of extracted DNA, with nuclease-free water accounting for the remaining volume. (4) Conclusions: This study contributes to the diversity and knowledge of Brazilian fresh-water turtle blood parasites, using integrative approaches for diagnosing and characterizing hemoparasites, with the identification of six undescribed species. Haemogregarina embaubali and six new species of Haemogregarina were observed and formally described as follows: H. (3) Results: A total of 40 turtles were screened and hemogregarines were observed in 34 (85%) individuals (3 Podocnemis expansa and 31 Podocnemis unifilis). Fragments of the organs (liver, spleen, heart, and kidney) were separated and stained with hematoxylin-eosin. Blood was stored at −20 ☌ for molecular analysis targeting the 18S rRNA gene. ![]() (2) Methods: In total, 2 mL of blood was collected, with between two to five blood smears prepared, fixed with absolute methanol, and stained with a 10% Giemsa solution. ![]() This study aimed to bring new insights on the diversity of species of Haemogregarina infecting Brazilian freshwater turtles from Mato Grosso and Goiás states using molecular and morphological tools. However, in Brazil, only three species have currently been characterised using molecular methods. (1) Background: Hemoparasites of the genus Haemogregarina (Haemogregarinidae) are commonly reported in freshwater turtles.
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